ARACHIDONIC-ACID INHIBITS GLYCINE TRANSPORT IN CULTURED GLIAL-CELLS

被引：49

作者：

ZAFRA, F ^{[1
]}

ALCANTARA, R ^{[1
]}

GOMEZA, J ^{[1
]}

ARAGON, C ^{[1
]}

GIMENEZ, C ^{[1
]}

机构：

[1] UNIV AUTONOMA MADRID,FAC CIENCIAS,CTR BIOL MOLEC,DEPT BIOL MOLEC,E-28049 MADRID,SPAIN

来源：

BIOCHEMICAL JOURNAL | 1990年 / 271卷 / 01期

关键词：

D O I：

10.1042/bj2710237

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of arachidonic acid on glycine uptake, exchange and efflux in C6 glioma cells were investigated. Arachidonic acid produced a dose-dependent inhibition of high-affinity glycine uptake. This effect was not due to a simple detergent-like action on membranes, as the inhibition of glycine transport was most pronounced with cis-unsaturated long-chain fatty acids, whereas saturated and trans-unsaturated fatty acids had relatively little or no effect. Endogenous unsaturated non-esterified fatty acid may exert a similar inhibitory effect on the transport of glycine. The mechanism for this inhibitory effect has been examined in a plasma membrane vesicle preparation derived from C6 cells, which avoids metabolic or compartmentation interferences. The results suggest that part of the selective inhibition of glycine transport by arachidonic acid could be due to the effects of the arachidonic acid on the lipid domain surrounding the carrier.

引用

页码：237 / 242

页数：6

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