BISINTERCALATORS OF DNA WITH A RIGID LINKER IN AN EXTENDED CONFIGURATION

被引：26

作者：

MULLINS, ST

ANNAN, NK

COOK, PR

LOWE, G

机构：

[1] UNIV OXFORD, DYSON PERRINS LAB, S PARKS RD, OXFORD OX1 3QY, ENGLAND

[2] UNIV OXFORD SIR WILLIAM DUNN SCH PATHOL, OXFORD OX1 3RE, ENGLAND

来源：

BIOCHEMISTRY | 1992年 / 31卷 / 03期

关键词：

D O I：

10.1021/bi00118a029

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new class of DNA bisintercalators is reported in which phenanthridinium or acridinium rings are connected by rigid and extended linkers of varied length. Cross-linking of DNA by bisintercalation is inferred from the unwinding and folding of linear DNA induced by the compound; after ligation and removal of the bisintercalator, superhelical circles, catenanes, and knots that bear an imprint of the bisintercalator are observed. These novel bisintercalators are of interest because they can be used to probe the organization of DNA in three-dimensional space, especially near sites of replication, recombination, or topoisomerase action, where two duplexes must be in close proximity.

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页码：842 / 849

页数：8

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