OBSERVATIONS ON THE DIRECT ELECTROCHEMISTRY OF BOVINE COPPER-ZINC SUPEROXIDE-DISMUTASE

被引：29

作者：

IYER, RN ^{[1
]}

SCHMIDT, WE ^{[1
]}

机构：

[1] GEORGE WASHINGTON UNIV,DEPT CHEM,WASHINGTON,DC 20052

来源：

BIOELECTROCHEMISTRY AND BIOENERGETICS | 1992年 / 27卷 / 03期

关键词：

D O I：

10.1016/0302-4598(92)87014-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cyclic voltammetric behavior of holo-superoxide dismutase (SOD) at pH 4.0 consists of an anodic peak at +0.270 V/SCE with no evidence of a cathodic feature on the forward (reduction) scan. Chronocoulometric data suggest that the reduced enzyme is strongly adsorbed in a close-packed arrangement. Background-subtracted differential pulse voltammograms yield poorly defined cathodic and anodic peaks at pH 5.0-7.0. Chemically reduced holo-SOD solutions exhibit markedly improved differential pulse voltammetric characteristics (especially the anodic), thus suggesting a conformational change occurring, possibly at the active site, consequent to electrochemical/chemical reduction that, in turn, enhances accessibility of the electrode to the active site.

引用

页码：393 / 404

页数：12

共 12 条

[1] VAN DER WAALS VOLUMES + RADII [J].