REPEATED FED-BATCH CULTURE OF HYBRIDOMA CELLS IN NUTRIENT-FORTIFIED HIGH-DENSITY MEDIUM

被引:15
作者
JO, EC
PARK, HJ
KIM, DI
MOON, HM
机构
[1] Mogam Biotechnology Research Institute, Kyounggi-Do, 449-910
[2] Department of Biological Engineering, Inha University, Incheon, 402-751, 253 Yonghyun-Dong, Nam-Ku
关键词
REPEATED FED-BATCH CULTURE; NUTRIENT FORTIFICATIONS; HIGH-DENSITY CULTURE; HYBRIDOMA;
D O I
10.1002/bit.260421013
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Long-term high-density cultivation of the hybridoma 2c3.1 was successfully carried out in a repeated fed-batch mode using high-density media that were constructed to meet in vitro cell growth limitations. The high-density culture was possible in a range of 0.5 approximately 1.0 x 10(7) cells/mL in MBRI 40-02 medium for over 2500 h by the repeated supplementation of the most fortified medium, MBRI 40-03, and consequently, distinct enhancement of MAb production was achieved. MAb concentrations were maintained around 1 g/L for about 1000 h of the process and the maximum MAb concentration was around 1.56 g/L. The result supported strongly the fact that the nutritional fortification was the most critical factor for high-density cell culture in vitro. The mean chromosome number of the hybridoma 2c3.1 was maintained stably for about 1500 h, whereas gradual loss of the MAb activity was apparent during the long-term cultivation. (C) 1993 John Wiley & Sons, Inc.
引用
收藏
页码:1229 / 1237
页数:9
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