AN IMPROVED METHOD TO CALCULATE ADIPOSE-TISSUE INTERSTITIAL SUBSTRATE RECOVERY FOR MICRODIALYSIS STUDIES

被引：43

作者：

JANSSON, PA ^{[1
]}

VENEMAN, T ^{[1
]}

NURJHAN, N ^{[1
]}

GERICH, J ^{[1
]}

机构：

[1] WHITTIER INST DIABET & ENDOCRINOL,LA JOLLA,CA 92037

来源：

LIFE SCIENCES | 1994年 / 54卷 / 21期

关键词：

MICRODIALYSIS; ADIPOSE TISSUE; SUBSTRATES;

D O I：

10.1016/0024-3205(94)90034-5

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

We simultaneously compared the conventional, time-consuming point of no net flux method for calculation of interstitial substrate recovery necessary for in vivo microdialysis studies with a simple isotopic method using rat epididymal fat pads. The recovery (%) calculated with the conventional method and the isotopic method for glucose (7.4 +/- 1.1 vs. 6.6 +/- 0.6), glycerol (23 +/- 4 vs. 26 +/- 5) and lactate (40 +/- 8 vs. 38 +/- 5), respectively, were not significantly different. Moreover, the overall correlation coefficient (N = 25) between the methods was 0.87, p < 0.001. We therefore conclude that the methods yield comparable results, and the more convenient isotopic method should become the method of choice for determining adipose tissue interstitial recovery for glucose, lactate and glycerol.

引用

页码：1621 / 1624

页数：4

共 11 条

[1] STEADY-STATE THEORY FOR QUANTITATIVE MICRODIALYSIS OF SOLUTES AND WATER INVIVO AND INVITRO [J].