MOLECULAR-CLONING OF 2 SPECIES OF CDNAS FOR HUMAN ALPHA-N-ACETYLGALACTOSAMINIDASE AND EXPRESSION IN MAMMALIAN-CELLS

被引：14

作者：

YAMAUCHI, T ^{[1
]}

HIRAIWA, M ^{[1
]}

KOBAYASHI, H ^{[1
]}

UDA, Y ^{[1
]}

MIYATAKE, T ^{[1
]}

TSUJI, S ^{[1
]}

机构：

[1] NIIGATA COLL PHARM,DEPT HLTH CHEM,NIIGATA 95021,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 170卷 / 01期

关键词：

D O I：

10.1016/0006-291X(90)91264-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two species of cDNAs for human α-N-acetylgalactosaminidase were isolated from a human fibroblast cDNA library. The two species differ each other by a 70 bp insertion in the coding region. Transient expression study in COS cells demonstrated that only the cDNA without the 70 bp insertion expressed α-N-acetylgalactosaminidase activity. Analysis of mRNA species utilizing polymerase chain reaction revealed that the majority of the mRNA does not contain the 70 bp insertion, and the mRNA containing the 70 bp insertion is present only in a minor amount in human brain. © 1990.

引用

页码：231 / 237

页数：7

共 18 条

[1]

BEUTLER E, 1972, J BIOL CHEM, V247, P7195

[2] BUFFER GRADIENT GELS AND S-35 LABEL AS AN AID TO RAPID DNA-SEQUENCE DETERMINATION [J].

BIGGIN, MD ;

GIBSON, TJ ;

HONG, GF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (13) :3963-3965

[3] STRUCTURAL ORGANIZATION OF THE HUMAN ALPHA-GALACTOSIDASE A-GENE - FURTHER EVIDENCE FOR THE ABSENCE OF A 3' UNTRANSLATED REGION [J].

BISHOP, DF ;

KORNREICH, R ;

DESNICK, RJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (11) :3903-3907

[4] TRANSCRIPTION OF THE DYSTROPHIN GENE IN HUMAN-MUSCLE AND NON-MUSCLE TISSUES [J].

CHELLY, J ;

KAPLAN, JC ;

MAIRE, P ;

GAUTRON, S ;

KAHN, A .

NATURE, 1988, 333 (6176) :858-860

[5] HIGH-EFFICIENCY TRANSFORMATION OF MAMMALIAN-CELLS BY PLASMID DNA [J].

CHEN, C ;

OKAYAMA, H .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (08) :2745-2752

[6] SUPERCOIL SEQUENCING - A FAST AND SIMPLE METHOD FOR SEQUENCING PLASMID DNA [J].

CHEN, EY ;

SEEBURG, PH .

DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1985, 4 (02) :165-170

[7]

DESNICK RJ, 1989, METABOLIC BASIS INHE, P1751

[8]

HIRABAYASHI Y, 1990, J BIOL CHEM, V265, P1693

[9]

KANZAKI T, 1989, LANCET, V1, P875

[10]

KOBAYASHI H, IN PRESS J MOL NEURO

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