ANAEROBIC MICROBIAL-DEGRADATION OF ACRIDINE AND THE APPLICATION OF REMOTE FIBER SPECTROSCOPY TO MONITOR THE TRANSFORMATION PROCESS

Anaerobic microbial transformation of a polynuclear nitrogen heterocycle, acridine, was studied in laboratory microcosms with three different inocula: a stabilized, mixed culture growing on ferulic acid that was originally enriched from anaerobic sewage sludge, and sulfate‐reducing and methanogenic aquifer materials from two sites at a groundwater aquifer contaminated by landfill leachate. Acridine degradation was investigated under methanogenic, denitrifying and sulfate‐reducing conditions at concentrations of 1 to 6 μg/ml. Substrate degradation was followed using two standard analytical techniques (HPLC and GC‐MS) and a new, in situ remote fiber spectroscopic (RFS) technique. This RFS was used successfully to follow changes in concentration of acridine with time, which indicates the technique has a significant potential for monitoring the degradation process in environmental media. Acridine was degraded extensively in one to three weeks under each of the conditions studied. A range of heterocyclic, homocyclic aromatic and aliphatic intermediates was identified by GC‐MS analyses. On the basis of these compounds, a tentative route of anaerobic acridine transformation is proposed that begins with oxidation and proceeds through the common degradative route for oxidized aromatic compounds. Copyright © 1990 SETAC