BENZOIC-ACID DETERMINATION THROUGH COMPETITIVE-INHIBITION OF MEDIATED BIOELECTROCATALYSIS

被引：19

作者：

SMIT, MH ^{[1
]}

RECHNITZ, GA ^{[1
]}

机构：

[1] UNIV HAWAII, DEPT CHEM, HAWAII BIOSENSOR LAB, HONOLULU, HI 96822 USA

来源：

ELECTROANALYSIS | 1993年 / 5卷 / 9-10期

关键词：

BENZOIC ACID; BIOSENSOR; INHIBITION; TYROSINASE;

D O I：

10.1002/elan.1140050907

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A bioelectrochemical system for the sensing of benzoic acid is described. An enzyme-based oxygen electrode is constructed where the enzymatic consumption of oxygen by tyrosinase is monitored electrochemically through the mediator 1,2 naphthoquinone, 4-sulfonate' (NQS). The bioelectrocatalysis of oxygen can be electrochemically initiated and terminated and is sensitive to inhibition by benzoic acid in a concentration-dependent manner. Inhibition of catalytic current results from a competitive interaction of benzoic acid at the enzymatic docking site of NQS. This inhibition of catalytic activity is reversible, and full catalytic activity can be reinstated following the removal of benzoic acid from solution. The sensing of benzoic acid is dependent on the nature of the mediator used, occurring with NQS but not with ferricyanide. These studies provide support for the possibility that analytes can be targeted for detection in such inhibition-based sensing through the proper selection of mediator.

引用

页码：747 / 751

页数：5

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