MOLECULAR-CLONING OF 3 ISOFORMS OF THE L-TYPE VOLTAGE-DEPENDENT CALCIUM-CHANNEL BETA-SUBUNIT FROM NORMAL HUMAN HEART

被引：56

作者：

COLLIN, T ^{[1
]}

WANG, JJ ^{[1
]}

NARGEOT, J ^{[1
]}

SCHWARTZ, A ^{[1
]}

机构：

[1] UNIV CINCINNATI,COLL MED,DEPT PHARMACOL & CELL BIOPHYS,231 BETHESDA AVE,CINCINNATI,OH 45267

来源：

CIRCULATION RESEARCH | 1993年 / 72卷 / 06期

关键词：

BETA-SUBUNITS; CALCIUM CHANNELS; CDNA CLONING; HUMAN HEART;

D O I：

10.1161/01.RES.72.6.1337

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We have cloned and sequenced cDNAs for three isoforms of the L-type voltage-dependent calcium channel beta subunit isolated from a normal human heart cDNA library. One of these subunits, designated beta(a), hybridized with a 3.4-kb message, and the other two, designated beta(b) and beta(c), hybridized with a message of approximately 1.9 kb. The presence of both of these latter two messages in human heart was confirmed by polymerase chain reaction methodology. Considering the differences between beta(a) and beta(b)/beta(c), we find it likely that these messages may be encoded by two different gene sequences, beta(a) and beta(b)/beta(c), and that the beta(b)/beta(c) sequence can be alternatively spliced in the 209-260 region. The data suggest that the human heart presents a different pattern of beta subunit expression from that found in the rat and rabbit heart.

引用

页码：1337 / 1344

页数：8

共 16 条

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