CLONING AND NUCLEOTIDE-SEQUENCE OF A CDNA OF THE HUMAN ERYTHROCYTE NADPH-FLAVIN REDUCTASE

被引：23

作者：

CHIKUBA, K ^{[1
]}

YUBISUI, T ^{[1
]}

SHIRABE, K ^{[1
]}

TAKESHITA, M ^{[1
]}

机构：

[1] OITA MED UNIV,DEPT BIOCHEM,OITA 87955,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 198卷 / 03期

关键词：

D O I：

10.1006/bbrc.1994.1165

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA of human erythrocyte NADPH-flavin reductase was cloned from a λgt 11 human reticulocyte cDNA library by polymerase chain reaction using degenerate primers and followed by a plaque hybridization. The nucleotide sequence of the cDNA contains an open reading frame of 621 base pairs which encodes 206 amino acid residues including an initial methionine. The amino acid sequence deduced from the base sequence coincided well with peptide sequence determined for the purified human erythrocyte NADPH-flavin reductase. A homologous sequence to the FMN-binding site of flavodoxins was found at the amino-terminal region. © 1994 Acdamic Press. Inc.

引用

页码：1170 / 1176

页数：7

共 24 条

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