ENZYMATIC MECHANISM OF TRANSFER-RNA (M(5)U54)METHYLTRANSFERASE

被引：58

作者：

KEALEY, JT

GU, X

SANTI, DV

机构：

[1] UNIV CALIF SAN FRANCISCO,DEPT PHARMACEUT CHEM,SAN FRANCISCO,CA 94143

[2] UNIV CALIF SAN FRANCISCO,DEPT BIOCHEM & BIOPHYS,SAN FRANCISCO,CA 94143

来源：

BIOCHIMIE | 1994年 / 76卷 / 12期

关键词：

TRANSFER-RNA MODIFICATION; METHYLATION; COVALENT CATALYSIS; PROTEIN-RNA RECOGNITION;

D O I：

10.1016/0300-9084(94)90042-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

tRNA (m(5)U54)methyltransferase (RUMT) catalyzes the methylation of uridine 54 of transfer RNA by S-adenosyl-L-methionine. In this report, we present the enzymatic mechanism of RUMT, including the stereochemical course of the methylation reaction, and discuss RUMT-tRNA recognition. As part of its enzymatic mechanism, we postulate that RUMT catalyzes the disruption of RNA-RNA contacts. We also show that many nucleotide substitutions can be made in the T-loop of tRNA without affecting RUMT binding, indicating that the recognition of the T-loop by RUMT is not stringent.

引用

页码：1133 / 1142

页数：10

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