A PLASMODIUM-CHABAUDI ANTIGEN LOCATED IN THE PARASITOPHOROUS VACUOLE MEMBRANE

被引：16

作者：

FAVALORO, JM ^{[1
]}

CULVENOR, JG ^{[1
]}

ANDERS, RF ^{[1
]}

KEMP, DJ ^{[1
]}

机构：

[1] ROYAL MELBOURNE HOSP,WALTER & ELIZA HALL INST MED RES,MELBOURNE,VIC 3050,AUSTRALIA

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1993年 / 62卷 / 02期

基金：

英国医学研究理事会;

关键词：

PLASMODIUM CHABAUDI; ANTIGEN; CDNA LIBRARY; AG3008; PC24; PLASMODIUM FALCIPARUM CRA; PARASITOPHOROUS VACUOLE MEMBRANE;

D O I：

10.1016/0166-6851(93)90115-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to study antigens from the rodent malaria Plasmodium chabaudi, clones reacting with mouse hyperimmune serum were selected from an expression library of brood-stage P. chabaudi cDNAs in the vector pGEX-2T. Sixty-four such clones were shown to derive from 19 different P. chabaudi antigens. One of these, Antigen 3008 (Ag3008), has a predicted size of 17.5 kDa and an observed size of 24 kDa. It is located in the parasitophorous vacuole membrane of maturing parasites and in the dense granules of merozoites, The cDNA sequence predicts a highly charged molecule with an N-terminal signal sequence and a central transmembrane domain, but no tandem repeats. The sequence reported for Pc24, a previously identified but uncharacterized P. chabaudi protein, corresponds to part of the 3' untranslated region in the Ag3008 mRNA. Ag3008 has many features in common with the P. falciparum circumsporozoite protein-related antigen (CRA).

引用

页码：263 / 270

页数：8

共 21 条

[1]

Anders R F, 1984, Mol Biol Med, V2, P177

[2]

ANDERS RF, 1988, PROG ALLERGY, V41, P148

[3] PATTERNS OF ANTIGEN EXPRESSION IN ASEXUAL BLOOD STAGES AND GAMETOCYTES OF PLASMODIUM-FALCIPARUM [J].

BIANCO, AE ;

CREWTHER, PE ;

COPPEL, RL ;

STAHL, HD ;

KEMP, DJ ;

ANDERS, RF ;

BROWN, GV .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1988, 38 (02) :258-267

[4] A BLOOD STAGE ANTIGEN OF PLASMODIUM-FALCIPARUM SHARES DETERMINANTS WITH THE SPOROZOITE COAT PROTEIN [J].

COPPEL, RL ;

FAVALORO, JM ;

CREWTHER, PE ;

BURKOT, TR ;

BIANCO, AE ;

STAHL, HD ;

KEMP, DJ ;

ANDERS, RF ;

BROWN, GV .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (15) :5121-5125

[5] AFFINITY PURIFICATION OF HUMAN-ANTIBODIES DIRECTED AGAINST CLONED ANTIGENS OF PLASMODIUM-FALCIPARUM [J].

CREWTHER, PE ;

BIANCO, AE ;

BROWN, GV ;

COPPEL, RL ;

STAHL, HD ;

KEMP, DJ ;

ANDERS, RF .

JOURNAL OF IMMUNOLOGICAL METHODS, 1986, 86 (02) :257-264

[6] PLASMODIUM-FALCIPARUM RING-INFECTED ERYTHROCYTE SURFACE-ANTIGEN IS RELEASED FROM MEROZOITE DENSE GRANULES AFTER ERYTHROCYTE INVASION [J].

CULVENOR, JG ;

DAY, KP ;

ANDERS, RF .

INFECTION AND IMMUNITY, 1991, 59 (03) :1183-1187

[7] CLONING OF CDNAS THAT CODE FOR PLASMODIUM-CHABAUDI ANTIGENS OF THE ERYTHROCYTIC FORMS AND CROSS-HYBRIDIZE WITH P-FALCIPARUM [J].

DASILVEIRA, JF ;

SIBILLI, L ;

BRUNET, E ;

MERCEREAUPUIJALON, O .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1984, 11 (APR) :133-143

[8]

HOLLOWAY SP, 1989, MOL MICROBIOL, V1, P1501

[9] THE GENE FOR AN EXPORTED ANTIGEN OF THE MALARIA PARASITE PLASMODIUM-FALCIPARUM CLONED AND EXPRESSED IN ESCHERICHIA-COLI [J].

HOPE, IA ;

MACKAY, M ;

HYDE, JE ;

GOMAN, M ;

SCAIFE, J .

NUCLEIC ACIDS RESEARCH, 1985, 13 (02) :369-379

[10] INHIBITORY MONOCLONAL-ANTIBODY AGAINST A (MYRISTYLATED) SMALL-MOLECULAR-WEIGHT ANTIGEN FROM PLASMODIUM-FALCIPARUM ASSOCIATED WITH THE PARASITOPHOROUS VACUOLE MEMBRANE [J].

KARA, UAK ;

STENZEL, DJ ;

INGRAM, LT ;

BUSHELL, GR ;

LOPEZ, JA ;

KIDSON, C .

INFECTION AND IMMUNITY, 1988, 56 (04) :903-909

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