AIRWAY AND PULMONARY TISSUE RESPONSES TO PLATELET-ACTIVATING-FACTOR IN RATS

We studied airway and pulmonary tissue responses to platelet-activating factor (PAF) in rats by measuring alveolar pressure with the alveolar capsule technique. We calculated airway resistance (Raw), dynamic elastance (Edyn), and pulmonary tissue resistance (Rtis). PAF was administered intravenously in doses of 0.1, 1, 10, and 30 mu g (1 dose per animal, 5 rats for each dose). Infusion of PAF resulted in a significant increase in Rtis and Edyn (p < .05). Maximal values of Edyn were observed with the infusion of 10 mu g of PAF (p < .02). Rtis presented a significant increase after infusion of 1 mu g of PAF (p < .05). The observed increase in Raw was transient and did not reach statistical significance. After infusion of PAF lungs were fixed by a quick-freezing method. Morphometric analysis showed that PAF infusion resulted in significant increases in intraluminal secretion (p = .001) and peribronchiolar edema (p < .001) but did not result in significant airway contraction. We conclude that intravenous infusion of PAF results in significant effects on lung tissue mechanics. The effects of PAF on the mechanical properties of lung parenchyma reflect the inflammatory alterations induced by this agonist in distal airspaces.