CLONING AND SEQUENCING OF THE ORNITHINE CARBAMOYLTRANSFERASE GENE FROM PACHYSOLEN-TANNOPHILUS

被引：5

作者：

SKRZYPEK, M ^{[1
]}

BORSUK, P ^{[1
]}

MALESZKA, R ^{[1
]}

机构：

[1] UNIV WARSAW,DEPT GENET,PL-00478 WARSAW,POLAND

来源：

YEAST | 1990年 / 6卷 / 02期

关键词：

ornithine carbamoyltransferase; Pachysolen; xylose; yeast;

D O I：

10.1002/yea.320060208

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A fragment of DNA from a yeast Pachysolen tannophilus, bearing the ornithine carbamoyltransferase gene (OCTase, EC 2.1.3.3) has been cloned from a genomic library by functional complementation of the Escherichia coli OCT‐negative mutant. The gene was located within the cloned segment of DNA and its coding sequence identified by DNA sequencing. This has indicated that P. tannophilus OCT gene encodes a 347 amino acid polypeptide, which shows 60% identity to the homologous Saccharomyces cerevisiae protein. The amino acid composition of its N‐terminus indicates that this protein is translocated across the mitochondrial membrane. The gene can be expressed in E. coli as well as in S. cerevisiae. Comparison with other OCTases confirms a high degree of conservation among these proteins. Copyright © 1990 John Wiley & Sons Ltd.

引用

页码：141 / 148

页数：8

共 31 条

[1] CLONING AND CHARACTERIZATION OF THE ORNITHINE CARBAMOYLTRANSFERASE GENE FROM ASPERGILLUS-NIDULANS
BERSE, B
DMOCHOWSKA, A
SKRZYPEK, M
WEGLENSKI, P
BATES, MA
WEISS, RL
[J]. GENE, 1983, 25 (01) : 109 - 117
[2] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[3] CLONING AND MOLECULAR ANALYSIS OF THE ORNITHINE CARBAMOYL TRANSFERASE GENE OF ASPERGILLUS-NIGER
BUXTON, FP
GWYNNE, DI
GARVEN, S
SIBLEY, S
DAVIES, RW
[J]. GENE, 1987, 60 (2-3) : 255 - 266
[4] IMPORT AND PROCESSING OF HUMAN ORNITHINE TRANSCARBAMOYLASE PRECURSOR BY MITOCHONDRIA FROM SACCHAROMYCES-CEREVISIAE
CHENG, MY
POLLOCK, RA
HENDRICK, JP
HORWICH, AL
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (12) : 4063 - 4067
[5] CLONING ARG3, THE GENE FOR ORNITHINE CARBAMOYLTRANSFERASE FROM SACCHAROMYCES-CEREVISIAE - EXPRESSION IN ESCHERICHIA-COLI REQUIRES SECONDARY MUTATIONS - PRODUCTION OF PLASMID BETA-LACTAMASE IN YEAST
CRABEEL, M
MESSENGUY, F
LACROUTE, F
GLANSDORFF, N
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (08): : 5026 - 5030
[6] CLONING AND ENDONUCLEASE RESTRICTION ANALYSIS OF ARGF AND THE CONTROL REGION OF THE ARGECBH BIPOLAR OPERON IN ESCHERICHIA-COLI
CRABEEL, M
CHARLIER, D
CUNIN, R
GLANSDORFF, N
[J]. GENE, 1979, 5 (03) : 207 - 231
[7] GENERAL AMINO-ACID CONTROL AND SPECIFIC ARGININE REPRESSION IN SACCHAROMYCES-CEREVISIAE - PHYSICAL STUDY OF THE BIFUNCTIONAL REGULATORY REGION OF THE ARG3 GENE
CRABEEL, M
HUYGEN, R
VERSCHUEREN, K
MESSENGUY, F
TINEL, K
CUNIN, R
GLANSDORFF, N
[J]. MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (11) : 3139 - 3148
[8] Cryer D R, 1975, Methods Cell Biol, V12, P39
[9] Cybis J, 1970, Bull Acad Pol Sci Biol, V18, P669
[10] COMPARTMENTAL AND REGULATORY MECHANISMS IN THE ARGININE PATHWAYS OF NEUROSPORA-CRASSA AND SACCHAROMYCES-CEREVISIAE
DAVIS, RH
[J]. MICROBIOLOGICAL REVIEWS, 1986, 50 (03) : 280 - 313

← 1 2 3 4 →