AMPEROMETRIC BIOSENSOR FOR RAPID MEASUREMENT OF 3-HYDROXYBUTYRATE IN UNDILUTED WHOLE-BLOOD AND PLASMA

被引：27

作者：

MCNEIL, CJ

SPOORS, JA

COOPER, JM

ALBERTI, KGMM

MULLEN, WH

机构：

[1] UNIV NEWCASTLE UPON TYNE,SCH MED,DEPT MED,NEWCASTLE TYNE NE2 4HH,ENGLAND

[2] CAMBRIDGE LIFE SCI PLC,ELY CB7 4DT,ENGLAND

来源：

ANALYTICA CHIMICA ACTA | 1990年 / 237卷 / 01期

基金：

英国医学研究理事会;

关键词：

Biosensor; Blood; Enzyme electrodes; Hydroxybutyrate; Plasma;

D O I：

10.1016/S0003-2670(00)83906-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple, rapid (< 30 s) electrochemical method for the determination of 3-hydroxybutyrate in whole blood or plasma is described, which uses NAD+-dependent d-3-hydroxybutyrate dehydrogenase immobilized at novel platinized carbon electrodes. The steady-state oxidation current produced by enzymatically generated NADH is measured at + 150 mV vs. Ag/AgCl. Enzyme electrodes produced by direct adsorption were stable for at least 3 months. Undiluted whole blood measurement with the sensor was compared with routine spectrophotometric analysis of plasma and perchloric acid extracts of whole blood. © 1990.

引用

页码：99 / 105

页数：7

共 21 条

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