IDENTIFICATION OF A HIGHLY CONSERVED REGION AT THE 5' FLANK OF THE PHOSPHOLAMBAN GENE

被引：19

作者：

JOHNS, DC ^{[1
]}

FELDMAN, AM ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV,SCH MED,DEPT MED,PETER BELFER LAB MOLEC BIOL HEART FAILURE,720 RUTLAND AVE,BALTIMORE,MD 21205

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 188卷 / 02期

关键词：

D O I：

10.1016/0006-291X(92)91144-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phospholamban is a protein that regulates the activity of the sarcoplasmic reticulum Ca2+-ATPase. The rat phospholamban gene contains a single intron of 6.5 kilobases which interrupts the 5′ untranslated region. Primer extension and nuclease mapping analysis identified a major transcription initiation site 87 nucleotides upstream of the first exon/intron junction. A highly conserved region was identified at the 5′ flank of the phospholamban gene. This region contained a TATA motif at position -52 which bound nuclear extract, and a consensus CHAT motif at position -76. This highly conserved region may be important in the regulation of basal transcriptional activity. © 1992.

引用

页码：927 / 933

页数：7

共 23 条

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