METABOLIC-ACTIVATION OF CHEMICALS TO MUTAGENIC CARCINOGENS BY HUMAN HEPATOMA MICROSOMAL EXTRACTS IN CHINESE-HAMSTER OVARY CELLS (INVITRO)

被引：43

作者：

DARROUDI, F ^{[1
]}

NATARAJAN, AT ^{[1
]}

机构：

[1] INTERUNIV INST,JA COHEN INST RADIOPATHOL & RADIAT PROTECT,LEIDEN,NETHERLANDS

来源：

MUTAGENESIS | 1993年 / 8卷 / 01期

关键词：

D O I：

10.1093/mutage/8.1.11

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The efficiency of human hepatoma (Hep G2) S9 microsomal fractions to activate indirectly acting genotoxic carcinogens was evaluated. The extract was prepared from Hep G2 epithelial cells, following sonication and centrifugation. The mutagenic activity of cyclophosphamide, benzo[a]pyrene, pyrene, hexamethylphosphoramide and safrole was assessed by the ability of their activated metabolites to induce sister chromatid exchange (SCE) and micronuclei (MN) in Chinese hamster ovary cells (CHO) (treated in vitro). All pro-mutagenic carcinogens tested were found to be effective only following metabolic activation by Hep G2 cell extracts. Non-carcinogen pyrene was not able to induce an increase in the frequencies of SCE or MN in CHO cells even in the presence of Hep G2 S9 microsomal fractions. Parallel experiments were carried out using rat liver homogenate (S9 fraction) as an exogenous activation system, and comparisons were made between these two in vitro systems and in vivo assays using the rodent.

引用

页码：11 / 15

页数：5

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