EVALUATION OF TRANSGENIC CANOLA PLANTS UNDER FIELD CONDITIONS

被引：18

作者：

ARNOLDO, M ^{[1
]}

BASZCZYNSKI, CL ^{[1
]}

BELLEMARE, G ^{[1
]}

BROWN, G ^{[1
]}

CARLSON, J ^{[1
]}

GILLESPIE, B ^{[1
]}

HUANG, B ^{[1
]}

MACLEAN, N ^{[1
]}

MACRAE, WD ^{[1
]}

RAYNER, G ^{[1
]}

ROZAKIS, S ^{[1
]}

WESTECOTT, M ^{[1
]}

KEMBLE, RJ ^{[1
]}

机构：

[1] UNIV LAVAL,DEPT BIOCHIM,QUEBEC CITY G1K 7P4,QUEBEC,CANADA

来源：

GENOME | 1992年 / 35卷 / 01期

关键词：

TRANSGENIC FIELD TRIAL; CANOLA; AGROBACTERIUM-MEDIATED TRANSFORMATION; VECTORS;

D O I：

10.1139/g92-010

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Eleven independent transgenic canola (Brassica napus ssp. oleifera L. cv. Westar and Regent) lines were evaluated in the field. The plants carried a neomycin phosphotransferase (NPTII) gene for kanamycin resistance that was introduced via Agrobacterium-mediated transformation. NPTII enzyme assays, Southern blot hybridizations and progeny analysis, confirmed the stable, heritable integration and expression of the introduced NPTII gene. A number of agronomic characteristics evaluated under field conditions, including maturity, yield, and oil and protein content, were all statistically comparable between the transformed and nontransformed plants. These results indicate that canola can be genetically engineered successfully, and that the Agrobacterium-based transformation system employed does not induce any adverse effects on the intrinsic agronomic and qualitative traits critical to the agricultural industry.

引用

页码：58 / 63

页数：6

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