YEAST SEC PROTEINS INTERACT WITH POLYPEPTIDES TRAVERSING THE ENDOPLASMIC-RETICULUM MEMBRANE

被引：109

作者：

MUSCH, A

WIEDMANN, M

RAPOPORT, TA

机构：

[1] Max-Delbrück-Center for Molecular Medicine, 1115 Berlin Buch

来源：

CELL | 1992年 / 69卷 / 02期

关键词：

D O I：

10.1016/0092-8674(92)90414-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We show by photocross-linking that nascent secretory proteins, during their passage through the endoplasmic reticulum membrane of S. cerevisiae, are in physical contact with Sec61p and Sec62p, two genetically identified membrane proteins that are essential for in vivo translocation. Sec61p seems to be in continuous contact, whereas Sec62p is involved only transiently. Translocation comprises both ATP-dependent and -independent phases of interaction with the Sec proteins. The results suggest a direct role of the Sec proteins in translocation.

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页码：343 / 352

页数：10

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