THE CHROMOSOMAL DNA OF STREPTOMYCES-LIVIDANS 66 IS LINEAR

被引：245

作者：

LIN, YS

KIESER, HM

HOPWOOD, DA

CHEN, CW

机构：

[1] NATL YANG MING MED COLL,INST GENET,TAIPEI,TAIWAN

[2] NATL YANG MING MED COLL,INST BIOCHEM,TAIPEI,TAIWAN

[3] JOHN INNES INST,JOHN INNES CTR,NORWICH NR4 7UH,NORFOLK,ENGLAND

来源：

MOLECULAR MICROBIOLOGY | 1993年 / 10卷 / 05期

关键词：

D O I：

10.1111/j.1365-2958.1993.tb00964.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two copies of a DNA sequence similar or identical to one end of the linear plasmid SLP2 were found on the Streptomyces lividans chromosome. Restriction mapping showed that these sequences represented free ends. Electrophoretic retardation and glass-binding studies indicated that the telomeres carry covalently bound proteins. Moreover, the chromosome migrated as an 8 Mb linear DNA in pulsed-field gel electrophoresis. A similar finding with the chromosomes of six other Streptomyces species suggested that a linear chromosome may be characteristic of the genus. The S. lividans chromosome can be circularized by joining the two ends by artificial targeted recombination or by spontaneous deletions spanning both telomeres. Thus the chromosome appears to be able to exist, in viable bacteria, as a linear or a circular molecule.

引用

页码：923 / 933

页数：11

共 46 条

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