THE WIDESPREAD EXPRESSION OF ANGIOGENIN IN DIFFERENT HUMAN-CELLS SUGGESTS A BIOLOGICAL FUNCTION NOT ONLY RELATED TO ANGIOGENESIS

被引:90
作者
MOENNER, M [1 ]
GUSSE, M [1 ]
HATZI, E [1 ]
BADET, J [1 ]
机构
[1] UNIV PARIS 12,RECH CROISSANCE CELLULAIRE LAB,CNRS,U1813,INSERM,F-94010 CRETEIL,FRANCE
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1994年 / 226卷 / 02期
关键词
D O I
10.1111/j.1432-1033.1994.tb20073.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Angiogenin is a secreted polypeptide that induces neovascularization in vivo. The expression of angiogenin by human cells in culture was investigated by using a specific radioimmunoassay and by cDNA hybridization. Angiogenin immunoreactivity was widely but differentially produced by anchorage-dependent growing cells including vascular endothelial cells from saphenous and umbilical veins, aortic smooth muscle cells, fibroblasts (from embryos, new-borns and adults), and tumour cells. Endothelial cells from saphenous veins and the endothelium-derived EA.hy926 cell line released immunoreactivity whatever the stage of the culture, including release at the lag phase, during exponential growth and at the confluent phase. However, the rate of accumulation of angiogenin varied as a function of EA.hy926 cell density. As compared to anchored cells, normal peripheral blood cells and tumour cells of myelomonocytic and megakaryocytic origin did not noticeably secrete angiogenin except at low levels. A myeloma cell line supernatant contained as much angiogenin cross-reactivity as did anchored cells, while four tumour T-cell lines expressed the crossreactivity at different levels, i.e. from undetectable levels to a high level. A 0.9-kb angiogenin messenger RNA was detected by Northern-blot analyses in a variety of representative cells correlating with the presence of immunoreactivity in the cell-culture media. The widespread expression pattern of angiogenin suggests a physiological function that is not restricted to the neovascularization process.
引用
收藏
页码:483 / 490
页数:8
相关论文
共 44 条
[1]   PURIFICATION AND PROPERTIES OF ALKALINE RIBONUCLEASE FROM HUMAN-SERUM [J].
AKAGI, K ;
MURAI, K ;
HIRAO, N ;
YAMANAKA, M .
BIOCHIMICA ET BIOPHYSICA ACTA, 1976, 442 (03) :368-378
[2]  
AUFFRAY C, 1980, EUR J BIOCHEM, V107, P303
[3]  
Badet J, 1990, Blood Coagul Fibrinolysis, V1, P721
[4]   SPECIFIC BINDING OF ANGIOGENIN TO CALF PULMONARY-ARTERY ENDOTHELIAL-CELLS [J].
BADET, J ;
SONCIN, F ;
GUITTON, JD ;
LAMARE, O ;
CARTWRIGHT, T ;
BARRITAULT, D .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (21) :8427-8431
[5]   INVITRO MODEL OF ANGIOGENESIS USING A HUMAN ENDOTHELIUM-DERIVED PERMANENT CELL-LINE - CONTRIBUTIONS OF INDUCED GENE-EXPRESSION, G-PROTEINS, AND INTEGRINS [J].
BAUER, J ;
MARGOLIS, M ;
SCHREINER, C ;
EDGELL, CJ ;
AZIZKHAN, J ;
LAZAROWSKI, E ;
JULIANO, RL .
JOURNAL OF CELLULAR PHYSIOLOGY, 1992, 153 (03) :437-449
[6]   AMINO-ACID SEQUENCE OF THE NONSECRETORY RIBONUCLEASE OF HUMAN-URINE [J].
BEINTEMA, JJ ;
HOFSTEENGE, J ;
IWAMA, M ;
MORITA, T ;
OHGI, K ;
IRIE, M ;
SUGIYAMA, RH ;
SCHIEVEN, GL ;
DEKKER, CA ;
GLITZ, DG .
BIOCHEMISTRY, 1988, 27 (12) :4530-4538
[7]   MOLECULAR EVOLUTION OF THE RIBONUCLEASE SUPERFAMILY [J].
BEINTEMA, JJ ;
SCHULLER, C ;
IRIE, M ;
CARSANA, A .
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, 1988, 51 (03) :165-192
[8]   ANGIOGENIN STIMULATES ENDOTHELIAL-CELL PROSTACYCLIN SECRETION BY ACTIVATION OF PHOSPHOLIPASE-A2 [J].
BICKNELL, R ;
VALLEE, BL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (05) :1573-1577
[9]   RIBONUCLEASES OF HUMAN-SERUM, URINE, CEREBROSPINAL-FLUID, AND LEUKOCYTES - ACTIVITY STAINING FOLLOWING ELECTROPHORESIS IN SODIUM DODECYL SULFATE-POLYACRYLAMIDE GELS [J].
BLANK, A ;
DEKKER, CA .
BIOCHEMISTRY, 1981, 20 (08) :2261-2267
[10]  
BLASER J, 1993, EUR J CLIN CHEM CLIN, V31, P513