SELECTIVE STABILITY-INDICATING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR RECOMBINANT HUMAN REGULAR INSULIN

被引：19

作者：

HOYER, GL ^{[1
]}

NOLAN, PE ^{[1
]}

LEDOUX, JH ^{[1
]}

MOORE, LA ^{[1
]}

机构：

[1] UNIV ARIZONA,UNIV HEART CTR,TUCSON,AZ 85721

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 699卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(95)00086-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

This report presents a selective HPLC assay capable of separating recombinant human regular insulin from insulin decomposition and transformation products. The assay utilizes an isocratic delivery of mobile phase, a C-18 peptide column, UV detection and is performed at ambient temperature. The standard curve ranges from 0.2 to 2.5 U/ml. The inter-day and intra-day variabilities are less than 7 and 5%, respectively, at the concentrations studied. The accuracy and precision are within 5% over the range of the standard curve.

引用

页码：383 / 388

页数：6

共 10 条

[1]

ASAHARA K, 1991, CHEM PHARM BULL, V39, P2662

[2]

LIQUID-CHROMATOGRAPHIC CONTROL OF THE IDENTITY, PURITY AND POTENCY OF BIOMOLECULES USED AS DRUGS [J].