USE OF THE OVEREXPRESSED BACILLUS-STEAROTHERMOPHILUS AMINOACYLASE FOR THE RESOLUTION OF D,L-AMINO ACIDS IN CONVENTIONAL AND NONCONVENTIONAL MEDIA

被引：7

作者：

DION, M

LOUSSOUARN, F

BATISSE, N

RABILLER, C

SAKANYAN, V

机构：

[1] UNIV NANTES, FAC SCI & TECH, BIOTECHNOL LAB, F-44072 NANTES 02, FRANCE

[2] UNIV NANTES, FAC SCI & TECH, RESONANCE MAGNET NUCL LAB, F-44072 NANTES 02, FRANCE

来源：

BIOTECHNOLOGY LETTERS | 1995年 / 17卷 / 09期

关键词：

D O I：

10.1007/BF00127424

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The B.stearothermophilus aminoacylase gene (ama) was overexpressed with the T7-expression vectors in Escherichia call. Crude extracts of the overexpressed strain efficiently catalyzed the resolution of N-acetyl-D,L-amino acids in the aqueous and the reverse reaction, acylation, in the vinyl acetate/triethylamine organic media,

引用

收藏

页码：905 / 910

页数：6

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