CHARACTERIZATION OF A 5'-FLANKING REGION OF THE HUMAN LIVER BONE KIDNEY ALKALINE-PHOSPHATASE GENE - 2 KINDS OF MESSENGER-RNA FROM A SINGLE GENE

被引：77

作者：

MATSUURA, S ^{[1
]}

KISHI, F ^{[1
]}

KAJII, T ^{[1
]}

机构：

[1] YAMAGUCHI UNIV, SCH MED, DEPT PEDIAT, UBE, YAMAGUCHI 755, JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 168卷 / 03期

关键词：

D O I：

10.1016/0006-291X(90)91127-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned the human liver/bone/kidney alkaline phosphatase (ALPL) gene using a liver-type ALPL cDNA as a probe. The gene is divided into 12 exons, and is likely to exist as a single copy in haploid genome. As compared with the gene isolated using a bone-type ALPL cDNA (Weiss et al., J. Biol. Chem. 263, 12002-12010, 1988), another leader exon specific for the liver-type ALPL mRNA was assigned about 3.4 kb upstream from exon 2 and the alternative splicing in the first exon was indicated. RNA blot analysis showed that three species or mRNA of 2.5,4.1 and 4.7 kilobases were detected in liver and developmentally regulated. © 1990.

引用

页码：993 / 1000

页数：8

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