C-TERMINAL PROCESSING OF BACILLUS-SUBTILIS BSE616 ENDO-BETA-1,4-GLUCANASE IN BACILLUS-MEGATERIUM

被引：13

作者：

KIM, H ^{[1
]}

KIM, SMF ^{[1
]}

PACK, MY ^{[1
]}

机构：

[1] KOREA INST SCI & TECHNOL, DEPT BIOL SCI & ENGN, SEOUL 130650, SOUTH KOREA

来源：

BIOTECHNOLOGY LETTERS | 1991年 / 13卷 / 11期

关键词：

D O I：

10.1007/BF01026762

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A large form of endo-beta-1,4-glucanase (endoglucanase) encoded by Bacillus subtilis BSE616 gene in B. megaterium (pCK98) was exocellularly produced at early log phase. The C-terminal portion of the secreted enzyme of 52 kilodaltons (Kd) was gradually cleaved and then converted to the smallest product of 33 Kd. N-terminal amino acid residues of both 52 Kd and 33 Kd enzyme were determined to be the same, Ala, the 30th residue of the primary translation product. Cleavage of the C-terminal portion increased specific and molecular activity of the enzyme.

引用

页码：799 / 804

页数：6

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