TRYPSIN-LIKE ARGININE AMIDASES INCLUDING PLASMINOGEN AND PLASMIN IN HUMAN SEMINAL PLASMA BY AFFINITY ADSORPTION AND ELUTION

被引：19

作者：

KOBAYASHI, T

MATSUDA, Y

PARK, JY

HARA, I

KANEKO, S

FUJIMOTO, Y

NOZAWA, S

AKIHAMA, S

机构：

[1] MEIJI COLL PHARM,DEPT BIOCHEM 1,SETAGAYA KU,TOKYO 154,JAPAN

[2] KEIO UNIV,SCH MED,DEPT OBSTET & GYNECOL,SHINANOMACHI,SHINJIKU KU,TOKYO 156,JAPAN

[3] HOKKAIDO INST PHARMACEUT SCI,DEPT CLIN BIOCHEM,OTARU,HOKKAIDO 04702,JAPAN

来源：

ARCHIVES OF ANDROLOGY | 1992年 / 28卷 / 03期

关键词：

SEMINAL PLASMA; PLASMINOGEN AND PLASMIN; TRYPSIN-LIKE ENZYME; ACIDIC ARGININE AMIDASE; BASIC ARGININE AMIDASE; AFFINITY ADSORPTION; ELUTION; ENZYME;

D O I：

10.3109/01485019208987694

中图分类号：

R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

摘要：

An enzyme preparation with affinity to a lysine column was detected from a DEAE-cellulose-adsorbed preparation of human seminal plasma containing plasminogen and plasmin. Two kinds of trypsin-like acidic arginine amidase activity with different affinity to lima bean trypsin inhibitor (LBTI) and aprotinin affinity column were detected from the DEAE-cellulose-adsorbed preparation after treatment of the lysine column. Two kinds of trypsin-like basic arginine amidase activity were also separated by the above-mentioned affinity adsorptions from a CM-cellulose-adsorbed preparation of human seminal plasma. The effect of calcium chloride on these two enzymes was different from human acrosin.

引用

页码：165 / 170

页数：6

共 16 条

[1]

Amundsen E, 1979, Adv Exp Med Biol, V120A, P83

[2] PURIFICATION OF BOVINE AND HUMAN ACROSIN [J].

ELCE, JS ;

MCINTYRE, EJ .

CANADIAN JOURNAL OF BIOCHEMISTRY, 1982, 60 (01) :8-14

[3]

FIEDLER F, 1979, BRADYKININ KALLIDIN, P103

[4]

Fink E, 1983, Adv Exp Med Biol, V156 (Pt B), P1175

[5]

GEIGER R, 1981, H-S Z PHYSIOL CHEM, V362, P1279

[6] PURIFICATION OF HUMAN-SPERM BY A DISCONTINUOUS PERCOLL DENSITY GRADIENT WITH AN INNERCOLUMN [J].

KANEKO, S ;

OSHIO, S ;

KOBANAWA, K ;

KOBAYASHI, T ;

MOHRI, H ;

IIZUKA, R .

BIOLOGY OF REPRODUCTION, 1986, 35 (04) :1059-1063

[7] STUDIES ON ACROSIN .1. PURIFICATION AND CHARACTERIZATION OF BOAR ACROSIN [J].

KANEKO, S ;

MORIWAKI, C .

JOURNAL OF PHARMACOBIO-DYNAMICS, 1981, 4 (01) :20-27

[8] HUMAN ACROSIN - PURIFICATION AND SOME PROPERTIES [J].

KOBAYASHI, T ;

MATSUDA, Y ;

OSHIO, S ;

KANEKO, S ;

NOZAWA, S ;

MHORI, H ;

AKIHAMA, S ;

FUJIMOTO, Y .

ARCHIVES OF ANDROLOGY, 1991, 27 (01) :9-16

[9]

KOBAYASHI T, 1990, MOL ANDROL, V3, P307

[10]

KOBAYASHI T, 1991, ARCH ANDROLOGY, V27, P196

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