EXPRESSION AND MUTATIONAL ANALYSIS OF THE NUCLEOID-ASSOCIATED PROTEIN H-NS OF SALMONELLA-TYPHIMURIUM

被引:104
作者
HINTON, JCD
SANTOS, DS
SEIRAFI, A
HULTON, CSJ
PAVITT, GD
HIGGINS, CF
机构
[1] Imperial Cancer Research Fund Laboratories, Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford
关键词
D O I
10.1111/j.1365-2958.1992.tb01408.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The H-NS (H1) protein is a major component of bacterial chromatin. Mutations in the hns (osmZ) gene encoding H-NS are highly pleiotropic, affecting the expression of many unrelated genes in an allele-specific manner. H-NS expression was found not to vary with growth phase or growth medium osmolarity. Additionally, 10 independent hns mutations were isolated and characterized. Five of these mutations were the result of an IS 10 insertion, each generating a truncated polypeptide. The other five mutations were the same specific deletion of one amino acid, DELTA-Ala46. The various hns mutations exhibited different phenotypes and influenced DNA topology to variable extents. Implications for the mechanism by which H-NS influences gene expression are discussed.
引用
收藏
页码:2327 / 2337
页数:11
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