HEMATOPOIETIC PROGENITOR CELLS ARE RESISTANT TO DIMETHYL-SULFOXIDE TOXICITY

Background: A direct chemical toxicity of dimethyl sulfoxide (DMSO) to hematopoietic progenitor cells has been suggested. However, a recent study failed to corroborate these earlier findings. Thus, a series of experiments was undertaken to address this issue. Study Design and Methods: Bone marrow was collected from 18 donors and cryopreserved with 10 percent (vol/vol) DMSO. Aliquots of frozen bone marrow were thawed, diluted with ACD-A to 8 percent (vol/vol) DMSO, and allowed to remain in DMSO for up to 2 hours before mononuclear cells were plated for colony-forming assays. After 14 days in culture, burst-forming units-erythroid, colony-forming units-granulocyte/macrophage, and colony-forming units-granulacyte/erythrocyte/mac rophage/megakaryocyte colonies were enumerated. Results: There was no significant difference (p>0.5) seen in colony formation over the 2-hour exposure to DMSO. Conclusion: These results support and extend a previous study that bone marrow hematopoietic progenitor cells, including burst-forming units-erythroid, colony-forming units-granulocyte/macrophage, and colony-forming units-granulocyte/erythrocyte/macrophage/megakaryocyte are resistant to any toxic effects of 8- to 10-percent (vol/vol) DMSO during at least 2 hours of DMSO exposure.