LOCALIZATION OF BETA-GLUCOSIDASE IN ROOTS OF CICER-ARIETINUM (L) BY A FLUORESCENCE TECHNIQUE

被引：11

作者：

GIERSE, HD ^{[1
]}

BARZ, W ^{[1
]}

机构：

[1] UNIV MUNSTER, LEHRSTUHL BIOCHEM PFLANZEN, D-4400 MUNSTER, BUNDES REPUBLIK

来源：

PROTOPLASMA | 1976年 / 88卷 / 01期

关键词：

D O I：

10.1007/BF01280355

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The localization of .beta.-glucosidases in roots of garbanzo beans [C. arietinum] was investigated by a 1-step fluorescence optical procedure using 4-methylumbelliferyl-.beta.-D-glucoside. The well known azo-coupling test for the localization of glucosidases could not be applied in polyphenol containing plants. The results obtained with the new method were comparable with those described in other investigations and showed that .alpha.-glucosidases were absent from the root tissues investigated. The aryl-.beta.-glucosidases were mainly detected in the outer region of the cortex and quantitatively increased towards the root tip.

引用

页码：1 / 6

页数：6

共 9 条

[1] HISTOCHEMICAL LOCALIZATION OF BETA-GLYCOSIDASES IN ROOTS OF ZEA-MAYS .1. SIMULTANEOUS COUPLING AZO-DYE TECHNIQUE FOR LOCALIZATION OF BETA-GLUCOSIDASE AND BETA-GALACTOSIDASE [J].

ASHFORD, AE .

PROTOPLASMA, 1970, 71 (03) :281-&

[2] HISTOCHEMICAL LOCALIZATION OF BETA-GLYCOSIDASES IN ROOTS OF ZEA-MAYS .2. CHANGES IN LOCALIZATION AND ACTIVITY OF BETA-GLUCOSIDASE IN MAIN ROOT APEX [J].

ASHFORD, AE ;

MCCULLY, ME .

PROTOPLASMA, 1970, 71 (04) :389-&

[3]

BARZ W, 1967, Z NATURFORSCH PT B, VB 22, P627

[4] PRESENCE AND DISTRIBUTION OF ALPHA AND BETA GLUCOSIDASE IN ROOT TIP [J].

BENES, K ;

GEORGIEVA, YD ;

POLACKOVA, D .

BIOLOGIA PLANTARUM, 1973, 15 (02) :88-94

[5]

COHEN RB, 1952, J BIOL CHEM, V195, P607

[6]

HOESEL W, IN PRESS

[7] HISTOCHEMISTRY OF BETA-GLUCOSIDASE IN ISOLINES OF ZEA-MAYS SUSCEPTIBLE OR RESISTANT TO NORTHERN CORN LEAF BLIGHT [J].

MACE, ME .

PHYTOPATHOLOGY, 1973, 63 (02) :243-&

[8] HISTOCHEMICAL LOCALIZATION OF ENZYMES IN TAPINANTHUS BANGWENSIS AND ITS HOSTS - ALKALINE-PHOSPHATASE AND BETA-GLUCOSIDASE [J].

ONOFEGHARA, FA .

BOTANICAL GAZETTE, 1973, 134 (01) :39-46

[9]

PAGETHOMAS DP, 1970, SPECTROVISION, V24, P3

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