LABILE EXOCYCLIC AMINE PROTECTION OF NUCLEOSIDES IN DNA, RNA AND OLIGONUCLEOTIDE ANALOG SYNTHESIS FACILITATING N-DEACYLATION, MINIMIZING DEPURINATION AND CHAIN DEGRADATION

被引：86

作者：

SINHA, ND

DAVIS, P

USMAN, N

PEREZ, J

HODGE, R

KREMSKY, J

CASALE, R

机构：

[1] ISIS PHARMACEUT,CARLSBAD,CA 92008

[2] RIBOZYME PHARMACEUT,BOULDER,CO 80301

来源：

BIOCHIMIE | 1993年 / 75卷 / 1-2期

关键词：

CHEMICAL SYNTHESIS OF DNA; RNA; CHIMERIC OLIGONUCLEOTIDES; SYNTHETIC RIBOZYME; OLIGONUCLEOTIDE METHYLPHOSPHONATE DIESTERS;

D O I：

10.1016/0300-9084(93)90019-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of tert-butylphenoxyacetyl for N-protection of nucleoside bases during oligonucleotide synthesis facilitates its removal under mild conditions. This protecting group reduces depurination of deoxyadenosine residues, and minimizes premature desilylation resulting in chain degradation during post-synthesis workup of synthetic RNA.

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页码：13 / 23

页数：11

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