CLONING AND CHARACTERIZATION OF NEISSERIA-MENINGITIDIS GENES ENCODING THE TRANSFERRIN-BINDING PROTEINS TBP1 AND TBP2

被引:131
作者
LEGRAIN, M [1 ]
MAZARIN, V [1 ]
IRWIN, SW [1 ]
BOUCHON, B [1 ]
QUENTINMILLET, MJ [1 ]
JACOBS, E [1 ]
SCHRYVERS, AB [1 ]
机构
[1] UNIV CALGARY, DEPT MICROBIOL & INFECT DIS, CALGARY T2N 4N1, AB, CANADA
关键词
RECOMBINANT DNA; TRANSFERRIN RECEPTOR; IRON; GRAM(-) BACTERIA; SIGNAL PEPTIDE; LIPOPROTEIN; TBP GENES;
D O I
10.1016/0378-1119(93)90348-7
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Genes tbp1 and tbp2, encoding the transferrin-binding proteins Tbp1 and Tbp2, have been isolated from two strains of Neisseria meningitidis. The tbp2 and tbp1 open reading frames are tandemly arranged in the genome with an 87-bp intergenic region, and the DNA region upstream from the tbp2-coding sequence contains domains homologous to Escherichia coli promoter consensus motives. Nucleotide sequence analysis suggests the existence of a Tbp1 precursor carrying an N-terminal signal peptide with a peptidase I cleavage site and of a Tbp2 precursor with N-terminal homology to lipoproteins, including a peptidase II cleavage site. Comparison of the Tbp1 deduced amino acid (aa) sequences from both strains showed about 76% aa homology, while those of Tbp2 revealed only about 47% aa homology. These comparisons should be extended to other Neisseria strains in order to evaluate further this genetic divergence further.
引用
收藏
页码:73 / 80
页数:8
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