CLONING AND CHARACTERIZATION OF NEISSERIA-MENINGITIDIS GENES ENCODING THE TRANSFERRIN-BINDING PROTEINS TBP1 AND TBP2

被引：131

作者：

LEGRAIN, M ^{[1
]}

MAZARIN, V ^{[1
]}

IRWIN, SW ^{[1
]}

BOUCHON, B ^{[1
]}

QUENTINMILLET, MJ ^{[1
]}

JACOBS, E ^{[1
]}

SCHRYVERS, AB ^{[1
]}

机构：

[1] UNIV CALGARY, DEPT MICROBIOL & INFECT DIS, CALGARY T2N 4N1, AB, CANADA

来源：

GENE | 1993年 / 130卷 / 01期

关键词：

RECOMBINANT DNA; TRANSFERRIN RECEPTOR; IRON; GRAM(-) BACTERIA; SIGNAL PEPTIDE; LIPOPROTEIN; TBP GENES;

D O I：

10.1016/0378-1119(93)90348-7

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Genes tbp1 and tbp2, encoding the transferrin-binding proteins Tbp1 and Tbp2, have been isolated from two strains of Neisseria meningitidis. The tbp2 and tbp1 open reading frames are tandemly arranged in the genome with an 87-bp intergenic region, and the DNA region upstream from the tbp2-coding sequence contains domains homologous to Escherichia coli promoter consensus motives. Nucleotide sequence analysis suggests the existence of a Tbp1 precursor carrying an N-terminal signal peptide with a peptidase I cleavage site and of a Tbp2 precursor with N-terminal homology to lipoproteins, including a peptidase II cleavage site. Comparison of the Tbp1 deduced amino acid (aa) sequences from both strains showed about 76% aa homology, while those of Tbp2 revealed only about 47% aa homology. These comparisons should be extended to other Neisseria strains in order to evaluate further this genetic divergence further.

引用

页码：73 / 80

页数：8

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