IMPROVEMENT IN HLA-DQB TYPING BY PCR-RFLP - INTRODUCTION OF A CONSTANT RESTRICTION SITE IN ONE OF THE PRIMERS FOR DIGESTION CONTROL

被引：23

作者：

MERCIER, B ^{[1
]}

FEREC, C ^{[1
]}

DUFOSSE, F ^{[1
]}

HUART, JJ ^{[1
]}

机构：

[1] CTR TRANSFUS SANGUINE & BIOGENET,HISTOCOMPATIBIL LAB,LILLE,FRANCE

来源：

TISSUE ANTIGENS | 1992年 / 40卷 / 02期

关键词：

DIGESTION CONTROL; HLA DQB ALLELES; PCR-RFLP; PRIMER;

D O I：

10.1111/j.1399-0039.1992.tb01964.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The PCR-RFLP method previously reported by Inoko is a powerful technique for HLA class II typing. The reliability of RFLP interpretation depends on complete digestion by restriction endonucleases using a modified primer with restriction sites as an internal digestion control. The use of restriction enzymes which recognize specific HLA DQB allelic variations makes HLA DQB genotyping possible.

引用

页码：86 / 89

页数：4

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