BIOSYNTHESIS OF 12(S)-HYDROXYEICOSATETRAENOIC ACID BY BOVINE CORNEAL EPITHELIUM

被引：18

作者：

OLIW, EH

机构：

[1] Department of Pharmaceutical Pharmacology, Uppsala University Biomedical Centre, Uppsala

来源：

ACTA PHYSIOLOGICA SCANDINAVICA | 1993年 / 147卷 / 01期

关键词：

CORNEA; CYTOCHROME-P-450; 12-LIPOXYGENASE; CHIRAL PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

D O I：

10.1111/j.1748-1716.1993.tb09479.x

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

Microsomes of bovine corneal epithelium metabolized [C-14]arachidonic acid extensively to [C-14]12-hydroxyeicosatetraenoic acid. The biosynthesis was not stimulated by exogenous NADPH and it could be inhibited by over 90% with two lipoxygenase inhibitors, esculetin and eicosatetraynoic acid. Chiral phase high performance liquid chromatography showed that 12-HETE consisted of the 12S enantiomer. The results indicate biosynthesis by 12S-lipoxygenase of bovine corneal epithelium rather than by cytochrome P450.

引用

页码：117 / 121

页数：5

共 23 条

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