BICARBONATE - CARBON-DIOXIDE REGULATION OF SPERM CAPACITATION, HYPERACTIVATED MOTILITY, AND ACROSOME REACTIONS

被引:138
作者
BOATMAN, DE [1 ]
ROBBINS, RS [1 ]
机构
[1] UNIV WISCONSIN,DEPT VET SCI,MADISON,WI 53715
关键词
D O I
10.1095/biolreprod44.5.806
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The bicarbonate: CO2 (HCO3-:CO2) concentration dependencies of hamster sperm motility, spontaneous acrosome reactions, and zona penetration (used to assay the zona-induced acrosome reaction) were examined. A cross-over experimental design was used to segregate effects on early stages of capacitation, spanning the first 5 h of incubation, from those on acrosome reactions and zona penetration during the last 1 h. After 5 h, HCO3-:CO2 concentrations were increased, decreased, or kept the same for 1 h. Compared to no HCO3-:CO2, as little as 2.9 mM:0.6% HCO3-:CO2 increased the sperm motility index (MI) by 2.7-3.6 times. When HCO3-:CO2 was continuously present, both progressive and hyperactivated motility were stimulated by HCO3-:CO2 in a dose-dependent manner by 3-4 h, well before completion of capacitation. Stimulation of acrosome reactions or zona penetration, by addition of HCO3-:CO2 to sperm for 1 h late in capacitation, depended mainly on levels of HCO3-:CO2 present earlier in capacitation. When 25 mM:5% HCO3-:CO2 was added only at 5 h, responses were significantly lower than with sperm treated continuously with the same concentration of HCO3-:CO2, being 2.5 times lower for MI, 2 times lower for acrosome reactions, and 6.3 times lower for zona penetration. In contrast, decreasing HCO3-:CO2 to suboptimal levels after 5 h did not decrease any 6-h sperm responses significantly. The average maximal and one-half maximal preincubation HCO3- concentrations for all responses were 34.2 +/- 1.0 and 9.2 +/- 0.3 mM, respectively. Zona penetration and hyperactivation were highly correlated. We conclude that 1) HCO3-:CO2 mainly affects sperm fertilizing ability by stimulating both motility and earlier stages of capacitation and that 2) acrosome reactions, either zona-induced or spontaneous, are only secondarily affected.
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页码:806 / 813
页数:8
相关论文
共 39 条
[1]   THE CAPACITATION OF THE MAMMALIAN SPERM [J].
AUSTIN, CR .
NATURE, 1952, 170 (4321) :326-326
[2]   POTASSIUM-DEPENDENT INCREASES IN CYTOSOLIC PH STIMULATE METABOLISM AND MOTILITY OF MAMMALIAN SPERM [J].
BABCOCK, DF ;
RUFO, GA ;
LARDY, HA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (05) :1327-1331
[3]   A RAPID SPERM MOTILITY BIOASSAY PROCEDURE FOR QUALITY-CONTROL TESTING OF WATER AND CULTURE MEDIA [J].
BAVISTER, BD ;
ANDREWS, JC .
JOURNAL OF IN VITRO FERTILIZATION AND EMBRYO TRANSFER, 1988, 5 (02) :67-75
[4]  
BAVISTER BD, 1974, J REPROD FERTIL, V38, P431, DOI 10.1530/jrf.0.0380431
[5]   A CONSISTENTLY SUCCESSFUL PROCEDURE FOR INVITRO FERTILIZATION OF GOLDEN-HAMSTER EGGS [J].
BAVISTER, BD .
GAMETE RESEARCH, 1989, 23 (02) :139-158
[6]   SIGNIFICANCE OF THE NEED FOR SPERM CAPACITATION BEFORE FERTILIZATION IN EUTHERIAN MAMMALS [J].
BEDFORD, JM .
BIOLOGY OF REPRODUCTION, 1983, 28 (01) :108-120
[7]   SYNTHETIC ORGANIC PH BUFFERS CAN SUPPORT FERTILIZATION OF GUINEA-PIG EGGS, BUT NOT AS EFFICIENTLY AS BICARBONATE BUFFER [J].
BHATTACHARYYA, A ;
YANAGIMACHI, R .
GAMETE RESEARCH, 1988, 19 (02) :123-129
[8]  
BOATMAN DE, 1983, J CELL BIOL, V97, pA12
[9]   A QUANTITATIVE ASSAY FOR CAPACITATION - EVALUATION OF MULTIPLE SPERM PENETRATION THROUGH THE ZONA PELLUCIDA OF SALT-STORED HAMSTER EGGS [J].
BOATMAN, DE ;
ANDREWS, JC ;
BAVISTER, BD .
GAMETE RESEARCH, 1988, 19 (01) :19-29
[10]  
BRACKETT BG, 1974, OVIDUCT ITS FUNCTION, P135