INHIBITION BY SPERMINE OF THE INDUCTION OF NITRIC-OXIDE SYNTHASE IN J774.2 MACROPHAGES - REQUIREMENT OF A SERUM FACTOR

被引：69

作者：

SZABO, C ^{[1
]}

SOUTHAN, GJ ^{[1
]}

WOOD, E ^{[1
]}

THIEMERMANN, C ^{[1
]}

VANE, JR ^{[1
]}

机构：

[1] UNIV LONDON ST BARTHOLOMEWS HOSP & MED COLL,WILLIAM HARVEY RES INST,LONDON EC1M 6BQ,ENGLAND

来源：

BRITISH JOURNAL OF PHARMACOLOGY | 1994年 / 112卷 / 02期

关键词：

NITRIC OXIDE; LIPOPOLYSACCHARIDE; SPERMINE; POLYAMINES;

D O I：

10.1111/j.1476-5381.1994.tb13078.x

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Polyamines are endogenous regulators of various cell functions. Nitric oxide (NO) is a cytostatic and cytotoxic free radical which is produced by the inducible NO synthase (iNOS) in immune-stimulated macrophages. We tested whether spermine modulates the induction of iNOS in J774.2 macrophages. Stimulation of macrophages by bacterial lipopolysaccharide (LPS) or gamma-interferon increased the accumulation of nitrite in the culture medium. Spermine (10(-6)-10(-4) M) inhibited nitrite production without causing cytotoxicity. This inhibition of NO formation by spermine was significantly reduced when it was given 6h after LPS. Spermine did not inhibit nitrite accumulation when foetal calf serum was omitted from the tissue culture medium. Thus, spermine is an inhibitor of the induction of iNOS, and its inhibitory activity requires the presence of a serum factor.

引用

页码：355 / 356

页数：2

共 8 条

[1] IMMUNE ASPECTS OF PATHOLOGY OF THE PLACENTAL BED CONTRIBUTING TO PREGNANCY PATHOLOGY [J].