HOMOGENEOUS IMMUNOASSAY OF POLYCLONAL ANTIBODIES BY USE OF ANTIGEN-COUPLED LIPOSOMES

被引：16

作者：

KATOH, S ^{[1
]}

SOHMA, Y ^{[1
]}

MORI, Y ^{[1
]}

FUJITA, R ^{[1
]}

SADA, E ^{[1
]}

KISHIMURA, M ^{[1
]}

FUKUDA, H ^{[1
]}

机构：

[1] KANEKA CORP, ENGN RES LABS, HYOGO 676, JAPAN

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1993年 / 41卷 / 09期

关键词：

HOMOGENEOUS IMMUNOASSAY; POLYCLONAL ANTIBODY; LYSIS OF LIPOSOME; COMPLEMENT SYSTEM;

D O I：

10.1002/bit.260410905

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Anti-cytochrome c and anti-myoglobin antibodies were assayed by use of immunoliposomes coupled with the antigens. Addition of complement under the existence of the antigen-antibody complex on the surface of the liposome caused lysis of the liposomes, which was proportional to the amount of the antigen-antibody complex formed as well as the concentration of complement added. Thus, the degree of marker release depended on the average association constant and also on its heterogeneity of the polyclonal antibodies, which shows that the results assayed by this method are correlated to the antibody ability to form the antigen-antibody complex.

引用

页码：862 / 867

页数：6

共 10 条

[1] MONOCLONAL-ANTIBODY COVALENTLY COUPLED TO LIPOSOMES - SPECIFIC TARGETING TO CELLS [J].