ANALYTICAL PROCEDURE FOR THE DETERMINATION OF RUFLOXACIN, A NEW PYRIDOBENZOTHIAZINE, IN HUMAN SERUM AND URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：12

作者：

CARLUCCI, G ^{[1
]}

PALUMBO, G ^{[1
]}

机构：

[1] UNIV LAQUILA,DIPARTIMENTO MED INTERNA & SANITA PUBBL,CATTEDRA FARMACOL,I-67100 LAQUILA,ITALY

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 564卷 / 01期

关键词：

D O I：

10.1016/0378-4347(91)80101-H

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic method for the quantification of rufloxacin in human serum and urine has been developed and validated. The compounds, rufloxacin and internal standard, are extracted from buffered serum and urine using dichloromethane. They are then separated on an anion-exchange column using 0.05 M phosphate buffer-acetonitrile (80:20, v/v). The eluate is quantified by measuring the ultraviolet absorbance at 296 nm. The lower limit of detection for the analyte is 0.1-mu-g/ml in serum and 0.05-mu-g/ml in urine. The method is linear from 0.3 to 10-mu-g/ml for serum and 0.1 to 10-mu-g/ml for urine. The method has been applied in a pharmacokinetic study in volunteers.

引用

页码：346 / 351

页数：6