A CHEMICALLY-DEFINED MEDIUM FOR ORGANOTYPIC SLICE CULTURES

被引：47

作者：

ANNIS, CM

EDMOND, J

ROBERTSON, RT

机构：

[1] UNIV CALIF LOS ANGELES,DEPT BIOL CHEM,LOS ANGELES,CA 90024

[2] UNIV CALIF LOS ANGELES,MENTAL RETARDAT RES CTR,LOS ANGELES,CA 90024

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1990年 / 32卷 / 01期

关键词：

Cerebral cortex; Defined medium; Development; Organotypic tissue culture;

D O I：

10.1016/0165-0270(90)90072-N

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Organotypic slice cultures provide an excellent system for the analysis of axonal connectivity and development. However, the study of the molecular mechanisms of this development necessitates the use of a chemically defined culture medium. We report here the development of a medium, EOL1 defined medium, designed specifically for this purpose. Cultures of both cerebral cortex and basal forebrain demonstrate that this defined medium allows a high degree of cytoarchitectural maintenance while promoting neural metabolism and process outgrowth. © 1990.

引用

页码：63 / 70

页数：8

共 23 条

[1] BORNSTEIN MB, 1958, LAB INVEST, V7, P134
[2] BRUNNER G, 1982, DEV BRAIN RES, V2, P563
[3] Crain S M, 1966, Int Rev Neurobiol, V9, P1, DOI 10.1016/S0074-7742(08)60135-X
[4] CYTOARCHITECTURE IN CULTURED RAT NEOCORTEX EXPLANTS
DEJONG, BM
RUIJTER, JM
ROMIJN, HJ
[J]. INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE, 1988, 6 (04) : 327 - &
[5] CAPACITY FOR SUBSTRATE UTILIZATION IN OXIDATIVE-METABOLISM BY NEURONS, ASTROCYTES, AND OLIGODENDROCYTES FROM DEVELOPING BRAIN IN PRIMARY CULTURE
EDMOND, J
ROBBINS, RA
BERGSTROM, JD
COLE, RA
DEVELLIS, J
[J]. JOURNAL OF NEUROSCIENCE RESEARCH, 1987, 18 (04) : 551 - 561
[6] ORGANOTYPIC MONOLAYER-CULTURES OF NERVOUS-TISSUE
GAHWILER, BH
[J]. JOURNAL OF NEUROSCIENCE METHODS, 1981, 4 (04) : 329 - 342
[7] DEVELOPMENT OF THE HIPPOCAMPUS INVITRO - CELL-TYPES, SYNAPSES AND RECEPTORS
GAHWILER, BH
[J]. NEUROSCIENCE, 1984, 11 (04) : 751 - 760
[8] ORGANOTYPIC CULTURES OF NEURAL TISSUE
GAHWILER, BH
[J]. TRENDS IN NEUROSCIENCES, 1988, 11 (11) : 484 - 489
[9] FUNCTIONAL INNERVATION OF CULTURED HIPPOCAMPAL-NEURONS BY CHOLINERGIC AFFERENTS FROM CO-CULTURED SEPTAL EXPLANTS
GAHWILER, BH
BROWN, DA
[J]. NATURE, 1985, 313 (6003) : 577 - 579
[10] KAGEYAMA GH, 1989, IN PRESS BRAIN RES

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