MOLECULAR-CLONING AND SEQUENCE-ANALYSIS OF CDNA-ENCODING DELTA-4-3-KETOSTEROID 5-BETA-REDUCTASE OF RAT-LIVER

被引：40

作者：

ONISHI, Y

NOSHIRO, M

SHIMOSATO, T

OKUDA, K

机构：

[1] HIROSHIMA UNIV,SCH DENT,DEPT BIOCHEM,KASUMI 1-2-3,MINAMI KU,HIROSHIMA 734,JAPAN

[2] HIROSHIMA UNIV,DEPT ORAL & MAXILLOFACIAL SURG 2,HIROSHIMA 734,JAPAN

来源：

FEBS LETTERS | 1991年 / 283卷 / 02期

关键词：

DELTA-4-3-KETOSTEROID; 5-BETA-REDUCTASE; CDNA CLONING;

D O I：

10.1016/0014-5793(91)80591-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA clone encoding DELTA-4-3-ketosteriod 5-beta-reductase was isolated from rat liver cDNA libraries using antibodies specific for the enzyme and oligonucleotides as probes. The cDNA contained 981-base pair open reading frame encoding 327 amino acid residues (M(r) 37 376) and an unusually long 3'-untranslated region rich in AT sequence in the total length of 3189 base pairs. The predicted amino acid sequence contains the sequences similar to the putative NADPH- and steroid-binding regions.

引用

页码：215 / 218

页数：4

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