RAPID HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR TOTAL HOMOCYSTEINE LEVELS IN HUMAN SERUM

被引：630

作者：

UBBINK, JB

VERMAAK, WJH

BISSBORT, S

机构：

[1] Department of Chemical Pathology, Faculty of Medicine, University of Pretoria, Pretoria, 0001

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 565卷 / 1-2期

关键词：

D O I：

10.1016/0378-4347(91)80407-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rapid, isocratic high-performance liquid chromatographic (HPLC) method is described for the determination of total homocysteine levels in human serum. Prior to reversed-phase HPLC analysis, the serum thiols were derivatized with SBD-F (ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulphonate), a thiol-specific fluorogenic probe which is commercially available. Retention of SBD-homocysteine was sensitive to pH, and a mobile phase pH of 2.1 ensured baseline separation of serum thiols within 6 min. The method is simple, sensitive, reproducible (between-run coefficient of variation of 6.6%) and very suitable for routine determination of serum homocysteine levels in a clinical pathology laboratory.

引用

页码：441 / 446

页数：6

共 24 条

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