ROLE OF PORPHYROMONAS-GINGIVALIS 40-KDA OUTER-MEMBRANE PROTEIN IN THE AGGREGATION OF P-GINGIVALIS VESICLES AND ACTINOMYCES-VISCOSUS

被引：52

作者：

HIRATSUKA, K ^{[1
]}

ABIKO, Y ^{[1
]}

HAYAKAWA, M ^{[1
]}

ITO, T ^{[1
]}

SASAHARA, H ^{[1
]}

TAKIGUCHI, H ^{[1
]}

机构：

[1] NIHON UNIV,SCH DENT MATSUDO,DEPT ORAL DIAGNOST,MATSUDO,CHIBA 271,JAPAN

来源：

ARCHIVES OF ORAL BIOLOGY | 1992年 / 37卷 / 09期

关键词：

PORPHYROMONAS-GINGIVALIS; OUTER MEMBRANE PROTEIN; AGGREGATION; COAGGREGATION; VESICLE; GENE CLONING;

D O I：

10.1016/0003-9969(92)90078-M

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Porphyromonas gingivalis, an important pathogen in periodontitis, produces extracellular vesicles that aggregate with Actinomyces viscosus cells. A 40-kDa outer membrane protein (OMP)-coding gene from P. gingivalis was cloned and the protein was found to be localized in these vesicles. The recombinant 40-kDa OMP did not show aggregation activity. However, affinity-purified antibody against the recombinant protein significantly inhibited aggregation of P. gingivalis vesicles with A. viscosus cells. The antibody also inhibited cellular coaggregation of several strains of P. gingivalis with A. viscosus cells, but not with other periodontal pathogens. Moreover, aggregation of A. viscosus cells with P. gingivalis vesicles was inhibited in a dose-dependent manner by pre-treatment of the A. viscosus cells with the recombinant protein. These findings suggest that the 40-kDa OMP may be an important aggregation factor of P. gingivalis.

引用

页码：717 / 724

页数：8

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