POTENT INHIBITION OF EPSTEIN-BARR-VIRUS BY PHOSPHOROTHIOATE OLIGODEOXYNUCLEOTIDES WITHOUT SEQUENCE SPECIFICATION

被引：24

作者：

YAO, GQ

GRILL, S

EGAN, W

CHENG, YC

机构：

[1] YALE UNIV,SCH MED,DEPT PHARMACOL,333 CEDAR ST,NEW HAVEN,CT 06510

[2] US FDA,CTR BIOL EVALUAT & RES,BIOPHYS LAB,BETHESDA,MD 20892

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1993年 / 37卷 / 07期

关键词：

D O I：

10.1128/AAC.37.7.1420

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We found that 28-mer phosphorothioate oligodeoxynucleotides (S-oligos) with and without sequence specificity complementary to Epstein-Barr virus (EBV) genes are potent inhibitors of EBV replication in cell culture. The decrease in the amount of EBV DNA, the activity of intracellular viral DNA polymerase, and virus production were dose dependent, with a 90% inhibitory dose of approximately 0.5 muM. No inhibition of cell growth was observed with the S-oligos at concentrations up to 20 muM. The mechanism of action appears to be the inhibition of EBV DNA synthesis. The reversibility of anti-EBV action is dependent on the dose and duration of drug exposure. S-oligos should be considered a new class of anti-EBV agents.

引用

页码：1420 / 1425

页数：6

共 27 条

[1] OLIGODEOXYNUCLEOSIDE PHOSPHORAMIDATES AND PHOSPHOROTHIOATES AS INHIBITORS OF HUMAN IMMUNODEFICIENCY VIRUS [J].