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APPLICATION OF THE POLYMERASE CHAIN-REACTION TO THE IDENTIFICATION OF ESCHERICHIA-COLI AND COLIFORMS IN WATER
被引:43
作者:
FRICKER, EJ
[1
]
FRICKER, CR
[1
]
机构:
[1] THAMES WATER UTILITIES,READING RG2 0JN,BERKS,ENGLAND
关键词:
D O I:
10.1111/j.1472-765X.1994.tb00900.x
中图分类号:
Q81 [生物工程学(生物技术)];
Q93 [微生物学];
学科分类号:
071005 ;
0836 ;
090102 ;
100705 ;
摘要:
The polymerase chain reaction (PCR) has been applied to the identification of Escherichia coli and coliforms after overnight growth using two sets of primers described previously. The primer set for E. coli, which was derived from the uid A gene, correctly identified all E. coli strains tested. The sequence was also identified in five non-E. coli coliforms. The coliform primer set correctly identified approximately 70% of the coliforms tested. We conclude that PCR can be used for the rapid identification of E. coli using the primers described here but that further work is required accurately to define a new primer set for the coliform group.
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页码:44 / 46
页数:3
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