TPA AND RESINIFERATOXIN-MEDIATED ACTIVATION OF NADPH-OXIDASE - A POSSIBLE ROLE FOR RX-KINASE AUGMENTATION OF PKC

被引：20

作者：

EVANS, AT ^{[1
]}

SHARMA, P ^{[1
]}

RYVES, WJ ^{[1
]}

EVANS, FJ ^{[1
]}

机构：

[1] UNIV LONDON,SCH PHARM,DEPT PHARMACOGNOSY,29-39 BRUNSWICK SQ,LONDON WC1N 1AX,ENGLAND

来源：

FEBS LETTERS | 1990年 / 267卷 / 02期

关键词：

Capsaicin; Mouse macrophage; NADPH-oxidase; Protein kinase C; Resiniferatoxin; Tetradecanoylphorbolacetate; Zymosan;

D O I：

10.1016/0014-5793(90)80938-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The non-tumour promoting irritant, resiniferatoxin, was capable of activating the NADPH-oxidase respiratory burst of starch-elicited, but not resident mouse peritoneal macrophages in vitro. Unlike TPA, the response was synergised by incubation with zymosan. The Rx-stimulated NADPH-oxidase activity in a cell-free assay was selectively enhanced in the presence of exogenous Rx-kinase rather than PKC and in the absence of Ca2+. Since resiniferatoxin is a poor activator of PKC, it is probable that the Ca2+-independent Rx-kinase plays a role in activation of the macrophage respiratory burst following stimulation by zymosan. © 1990.

引用

页码：253 / 256

页数：4