FERTILIZATION AND EMBRYONIC-DEVELOPMENT OF HUMAN OOCYTES AFTER COOLING

被引：18

作者：

BERNARD, A

HUNTER, JE

FULLER, BJ

IMOEDEMHE, D

CURTIS, P

JACKSON, A

机构：

[1] University of Wales College of Medicine, Department of Obstetrics and Gynaecology

[2] Departments of Obstetrics and Gynaecology

[3] Royal Free Hospital, School of Medicine

[4] Soliman Fakeeh Hospital, Jeddah

来源：

HUMAN REPRODUCTION | 1992年 / 7卷 / 10期

基金：

英国医学研究理事会;

关键词：

COLD SHOCK; CRYOPRESERVATION; HUMAN OOCYTES;

D O I：

10.1093/oxfordjournals.humrep.a137592

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Injury to living cells resulting from rapid cooling to temperatures at or near 0-degrees-C has long been recognized, and the phenomenon, which is termed 'cold shock', has been known to occur in some mammalian gametes. Although human embryos have been successfully stored at low temperatures, cryopreservation of the human oocyte is proving to be more difficult. Whether or not this lack of success is a direct result of cellular injury brought about by cold shock' is the purpose of the current investigation. Human oocytes were cooled, in the absence of cryoprotectants, at two different cooling rates (-3-degrees-C/min and -1000+-degrees-C/min) to a temperature of 0-degrees-C and rewarmed prior to insemination. In both cases fertilization after cooling was similar to the rates achieved in a routine in-vitro fertilization and embryo transfer procedure. After cooling at -3-degrees-C/min, the rate of fertilization was 19/22 (86%) and after cooling at -1000+-degrees-C/min, 9/9 (100%), with non-cooled control rates of 62/87 (71%) and 35150 (70%) respectively. Fertilized oocytes from both groups were successfully cultured for a further 24 h before termination of the experiment.

引用

页码：1447 / 1450

页数：4

共 27 条

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