THE CYTOPLASMIC TAIL OF THE NEURAMINIDASE PROTEIN OF INFLUENZA-A VIRUS DOES NOT PLAY AN IMPORTANT ROLE IN THE PACKAGING OF THIS PROTEIN INTO VIRAL ENVELOPES

被引：46

作者：

GARCIASASTRE, A

PALESE, P

机构：

[1] Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029

[2] On leave of absence from the Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Salamanca, Salamanca

来源：

VIRUS RESEARCH | 1995年 / 37卷 / 01期

基金：

美国国家卫生研究院;

关键词：

INFLUENZA VIRUS; VIRUS PACKAGING; VIRUS BUDDING; REVERSE GENETICS;

D O I：

10.1016/0168-1702(95)00017-K

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have rescued a transfectant influenza virus, NA/TAIL(-), whose neuraminidase (NA) protein lacks the predicted cytoplasmic tail. The virus was attenuated (one log(10) reduction) both in tissue culture and in mouse lungs. Attenuation correlated with a 50% reduction of the level of NA in infected cells and levels of incorporation of the tail-less NA protein into viral particles paralleled that in infected cells. This result indicates that the signal for packaging of the NA protein into the viral envelope is not located in its cytoplasmic domain.

引用

页码：37 / 47

页数：11

共 35 条

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