SEPARATION OF DERMATAN SULFATE FROM HEPARAN SULFATE IN MUCOPOLYSACCHARIDOSIS URINE BY CHROMATOGRAPHY ON SEPHADEX G-75

被引：2

作者：

HERD, JK ^{[1
]}

FORREST, T ^{[1
]}

TSCHIDA, J ^{[1
]}

机构：

[1] CREIGHTON UNIV, SCH MED, DEPT PEDIAT, OMAHA, NE 68131 USA

来源：

CLINICA CHIMICA ACTA | 1976年 / 68卷 / 01期

关键词：

D O I：

10.1016/0009-8981(76)90282-5

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Oligosaccharides of testicular hyaluronidase-degraded dermatan sulfate were separated from undegraded dermatan sulfate by chromatography on Sephadex G-75, but not by chromatography on Sephadex G-25. All but the smallest of these oligosaccharides were recovered in excellent yield following dialysis and precipitation with cetyl pyridinium chloride (CPC). G-75 chromatography of dialyzed, concentrated Hunter urine mucopolysaccharides precipitated with CPC resolved most of the large dermatan sulfate into a void volume related peak which was free of heparan sulfate. Decreasing amounts of dermatan sulfate oligosaccharides were eluted with the Sephadex-retarded polysaccharides, including small amounts which appeared with otherwise pure heparan sulfate.

引用

页码：1 / 9

页数：9

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