SELECTIVE INDUCTION OF BILIRUBIN UDP-GLUCURONOSYLTRANSFERASE BY PERFLUORODECANOIC ACID

被引：9

作者：

ARAND, M ^{[1
]}

COUGHTRIE, MWH ^{[1
]}

BURCHELL, B ^{[1
]}

OESCH, F ^{[1
]}

ROBERTSON, LW ^{[1
]}

机构：

[1] UNIV DUNDEE,INST MED SCI,DEPT BIOCHEM,DUNDEE DD1 4HN,SCOTLAND

来源：

CHEMICO-BIOLOGICAL INTERACTIONS | 1991年 / 77卷 / 01期

基金：

英国惠康基金;

关键词：

UDP-GLUCURONOSYLTRANSFERASES; BILIRUBIN GLUCURONIDATION; ENZYME INDUCTION; PERFLUORODECANOIC ACID; PEROXISOME PROLIFERATION;

D O I：

10.1016/0009-2797(91)90008-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Differential effects of perfluorodecanoic acid (PFDA) on rat liver UDP-glucuronosyltransferase isoenzymes have been observed after a single i.p. administration of the compound to young male Sprague-Dawley rats. (1) Bilirubin glucuronidation was induced 2-fold. The induced state was stable for at least 3 weeks. (2) Glucuronidation of 1-naphthol, morphine and testosterone was decreased to half of the control values. These decreases were maximal after 12 days but all three activities returned to normal levels after 3 weeks. (3) Immunoblotting experiments indicated that the differential effects of PFDA on UDP-glucuronosyltransferase activities were due to modulation of enzyme protein concentrations rather than activation/inactivation mechanisms. With respect to its influence on UDP-glucuronosyltransferase isoenzymes, PFDA may be classified as a clofibrate-type inducer. The persistence of the induction after a single application however is unique among peroxisome proliferators and therefore PFDA may be a useful, elective inducer of bilirubin glucuronidation.

引用

页码：97 / 105

页数：9

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