PROMOTERS OF AUXIN-INDUCED GENES FROM TOBACCO CAN LEAD TO AUXIN-INDUCIBLE AND ROOT TIP-SPECIFIC EXPRESSION

被引：98

作者：

VANDERZAAL, EJ ^{[1
]}

DROOG, FNJ ^{[1
]}

BOOT, CJM ^{[1
]}

HENSGENS, LAM ^{[1
]}

HOGE, JHC ^{[1
]}

SCHILPEROORT, RA ^{[1
]}

LIBBENGA, KR ^{[1
]}

机构：

[1] BOT LAB,2311 VJ LEIDEN,NETHERLANDS

来源：

PLANT MOLECULAR BIOLOGY | 1991年 / 16卷 / 06期

关键词：

AUXIN-INDUCED GENES; HISTOCHEMICAL LOCALIZATION; NICOTIANA-TABACUM; ROOT TIP-SPECIFIC EXPRESSION;

D O I：

10.1007/BF00016071

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In previous studies we have identified several mRNAs which accumulate after addition of 2,4-dichloro-phenoxyacetic-acid (2,4-D) to auxin-starved tobacco cells [45, 46]. The mRNAs corresponding to cDNA clone pCNT103 were found to accumulate transiently prior to the cell division response due to auxin treatment. In this study we determined the sequences of three 103-like cDNAs and two 103-like genes, GNT1 and GNT35. To further study the regulation of the expression of these genes their 5' regions were translationally fused with the beta-D-glucuronidase reporter gene (GUS). The GNT1 5' region led to GUS expression only in the root tips of transgenic plants. By using transgenic hairy-root cultures and transformed cell suspension cultures it was shown that the 5' regions of both GNT1 and GNT35 lead to 2,4-D-inducible expression of GUS activity. The homology of the 103-like genes with other auxin-regulated genes is evaluated.

引用

页码：983 / 998

页数：16

共 47 条

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