THE PRESENCE OF FREE D-ALANINE, D-PROLINE AND D-SERINE IN MICE

被引：81

作者：

NAGATA, Y

YAMAMOTO, K

SHIMOJO, T

KONNO, R

YASUMURA, Y

AKINO, T

机构：

[1] SAPPORO MED COLL,DEPT BIOCHEM,SAPPORO,HOKKAIDO 060,JAPAN

[2] OSAKA WOMENS UNIV,ORGAN CHEM LAB,SAKAI,OSAKA 590,JAPAN

[3] DOKKYO UNIV,SCH MED,DEPT MICROBIOL,MIBU,TOCHIGI 32102,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1115卷 / 03期

关键词：

D-ALANINE; D-PROLINE; D-SERINE; D-ISOMER; HPLC;

D O I：

10.1016/0304-4165(92)90055-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The presence of free D-alanine, D-proline and D-serine was demonstrated in mammalian tissues, using a mutant mouse strain lacking D-amino acid oxidase. In the experiment, free amino acids from the kidney and serum were derivatized with 1-fluoro-2,4-dinitrophenyl-5-L-alanine amide (FDAA) to diastereomers, separated by two-dimensional thin-layer chromatography (TLC), and analysed by reversed-phase high-performance liquid chromatography (HPLC) for the resolution of D- and L-isomers. D/L ratios of alanine, proline and serine were obtained based on the peak areas of HPLC.

引用

页码：208 / 211

页数：4

共 15 条

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